{"id":872,"date":"2019-04-03T00:45:35","date_gmt":"2019-04-02T15:45:35","guid":{"rendered":"https:\/\/mge.hiroshima-u.ac.jp\/SATREPS_MACH\/?p=872"},"modified":"2019-09-12T00:54:50","modified_gmt":"2019-09-11T15:54:50","slug":"jica%e3%83%81%e3%83%aa%e6%94%af%e6%89%80%e9%95%b7%e3%81%ab%e9%ae%ad%e6%8a%80%e8%a1%93%e7%a0%94%e7%a9%b6%e6%89%80%ef%bc%88intesal%ef%bc%89%e3%82%92%e8%a8%aa%e5%95%8f%e3%81%84%e3%81%9f%e3%81%a0-2","status":"publish","type":"post","link":"https:\/\/mge.hiroshima-u.ac.jp\/SATREPS_MACH\/en\/topics\/news\/20190403_872\/","title":{"rendered":"Field work was developed in the IFOP-CREAN facilities by MACH-SATREPS lab assistant Joaquin Rilling"},"content":{"rendered":"<p><\/p>\n<p>On March 5th (Tuesday) 2019 field work was developed in the IFOP-CREAN facilities by MACH-SATREPS lab assistant Mr. Joaquin Rilling (assigned to assist Dr. So Fujiyoshi in UFRO). The field work developed consisted in the detection of <em>Alexandrium catenella<\/em> in water DNA samples collected from Los Lagos Region through Loop-mediated isothermal DNA amplification (LAMP).<\/p>\n<p>The methodology applied required Mr. Rilling to travel personally to Puerto Montt to bring both the suitcase lab where the equipment (Kaneka LAMP detector) its contained, and to use it due to his expertise. In this opportunity, the DNA samples were provided directly by Dr. Kyoko Yarimizu, who detected the presence of <em>A. catenella<\/em> microscopically before the DNA extraction procedure.<\/p>\n<p>The results of the LAMP analysis confirmed the observed by Dr. Yarimizu, and also allowed the detection of the target microorganism in samples where microscopic evaluation did not reveal its presence. Complementarily, the field work experience allowed Mr. Rilling to provide LAMP training to Mr. Jonnathan Vilugron, Dr. Yarimizu\u2019s lab assistant for future proceedings, as well as to bring samples under cold storage to UFRO.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone  wp-image-876\" src=\"https:\/\/mge.hiroshima-u.ac.jp\/SATREPS_MACH\/wp-content\/uploads\/2019\/04\/IMG_2641-1024x768.jpg\" alt=\"\" width=\"387\" height=\"290\" srcset=\"https:\/\/mge.hiroshima-u.ac.jp\/SATREPS_MACH\/wp-content\/uploads\/2019\/04\/IMG_2641-1024x768.jpg 1024w, https:\/\/mge.hiroshima-u.ac.jp\/SATREPS_MACH\/wp-content\/uploads\/2019\/04\/IMG_2641-360x270.jpg 360w, https:\/\/mge.hiroshima-u.ac.jp\/SATREPS_MACH\/wp-content\/uploads\/2019\/04\/IMG_2641-300x225.jpg 300w, https:\/\/mge.hiroshima-u.ac.jp\/SATREPS_MACH\/wp-content\/uploads\/2019\/04\/IMG_2641-768x576.jpg 768w\" sizes=\"auto, (max-width: 387px) 100vw, 387px\" \/><\/p>\n<p>UFRO lab assistant Mr. Rilling (left) and IFOP lab assistant Mr. Vilugron (right)<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone  wp-image-877\" src=\"https:\/\/mge.hiroshima-u.ac.jp\/SATREPS_MACH\/wp-content\/uploads\/2019\/04\/2019.3.5LAPTOP-1024x576.jpg\" alt=\"\" width=\"395\" height=\"222\" srcset=\"https:\/\/mge.hiroshima-u.ac.jp\/SATREPS_MACH\/wp-content\/uploads\/2019\/04\/2019.3.5LAPTOP-1024x576.jpg 1024w, https:\/\/mge.hiroshima-u.ac.jp\/SATREPS_MACH\/wp-content\/uploads\/2019\/04\/2019.3.5LAPTOP-300x169.jpg 300w, https:\/\/mge.hiroshima-u.ac.jp\/SATREPS_MACH\/wp-content\/uploads\/2019\/04\/2019.3.5LAPTOP-768x432.jpg 768w, https:\/\/mge.hiroshima-u.ac.jp\/SATREPS_MACH\/wp-content\/uploads\/2019\/04\/2019.3.5LAPTOP.jpg 1280w\" sizes=\"auto, (max-width: 395px) 100vw, 395px\" \/><\/p>\n<p>LAMP detector with Mr. Rilling personal laptop<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"attachment-266x266 size-266x266\" src=\"https:\/\/mge.hiroshima-u.ac.jp\/SATREPS_MACH\/wp-content\/uploads\/2019\/04\/LAMP-e1554165797390-360x270.png\" alt=\"\" width=\"266\" height=\"200\" \/><\/p>\n<p>\uff08Left\uff09<em>A. catanella<\/em> positive\uff08Right\uff09Negative control<\/p>\n<p>Under UV light, visual fluorescence detection can be conducted (Left)<\/p>\n<p><\/p>","protected":false},"excerpt":{"rendered":"<p>On March 5th (Tuesday) 2019 field work was developed in the IFOP-CREAN facilities by MACH-SATREPS lab assistan [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":874,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[3,5],"tags":[],"class_list":["post-872","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-news","category-achievements"],"acf":[],"_links":{"self":[{"href":"https:\/\/mge.hiroshima-u.ac.jp\/SATREPS_MACH\/en\/wp-json\/wp\/v2\/posts\/872","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mge.hiroshima-u.ac.jp\/SATREPS_MACH\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/mge.hiroshima-u.ac.jp\/SATREPS_MACH\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/mge.hiroshima-u.ac.jp\/SATREPS_MACH\/en\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/mge.hiroshima-u.ac.jp\/SATREPS_MACH\/en\/wp-json\/wp\/v2\/comments?post=872"}],"version-history":[{"count":8,"href":"https:\/\/mge.hiroshima-u.ac.jp\/SATREPS_MACH\/en\/wp-json\/wp\/v2\/posts\/872\/revisions"}],"predecessor-version":[{"id":921,"href":"https:\/\/mge.hiroshima-u.ac.jp\/SATREPS_MACH\/en\/wp-json\/wp\/v2\/posts\/872\/revisions\/921"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/mge.hiroshima-u.ac.jp\/SATREPS_MACH\/en\/wp-json\/wp\/v2\/media\/874"}],"wp:attachment":[{"href":"https:\/\/mge.hiroshima-u.ac.jp\/SATREPS_MACH\/en\/wp-json\/wp\/v2\/media?parent=872"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/mge.hiroshima-u.ac.jp\/SATREPS_MACH\/en\/wp-json\/wp\/v2\/categories?post=872"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/mge.hiroshima-u.ac.jp\/SATREPS_MACH\/en\/wp-json\/wp\/v2\/tags?post=872"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}